determination of diazinon’s toxicity degree and evaluation its effecton some enzymatic indices in huso huso

نویسندگان

نیما شیری

دانش آموخته کارشناسی ارشد بوم شناسی آبزیان، گروه شیلات و محیط زیست، دانشکده منابع طبیعی، دانشگاه تهران. خدیجه خوشنودی فر

دانش آموخته کارشناسی ارشد تکثیر و پرورش آبزیان،گروه شیلات و محیط زیست، دانشکده منابع طبیعی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان. غلامرضا رفیعی

استاد گروه شیلات و محیط زیست، دانشکده منابع طبیعی، دانشگاه تهران علیرضا میرواقفی

دانشیار گروه شیلات و محیط زیست، دانشکده منابع طبیعی، دانشگاه تهران.

چکیده

in present study, it was determinated lc50 96-h and toxicity degree of diazinon in huso huso, then were evaluated effects of sub-lethal concentrations on tow general enzymatic indices including alanine and aspartate aminotransferase of plasma and one specific enzymatic index including tissue ache enzyme. the median lethal concentration, lowest observed effect concentration and maximum allowable toxicant concentration was measured 5.47, 4.28 and 0.54 mg.l-1, respectively. also, diazinon according by amount of lc50 was classified in “moderate toxicity degree” for this sturgeon. the test results showed that with increasing sub-lethal concentrations of diazinon in water, ast and alt enzyme activity in experimental fish were increased, thus, a direct relationship seen between the concentration of diazinon with the public indices. however, increased concentrations of diazinon, causing a decrease in cholinesterase activity in both brain and muscle tissues. in other words, inhibition of ache is directly related to the concentration of pesticide.the diazinon pesticide concentrations for non-target specieses which were ineffective and allowable according to the lethal test can lead to their enzymatic indicies of serum and ache responses.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Histomorphogenesis of cranial nerves in Huso huso larvae

In this study the cranial nerves development of H. huso are explained from 1 to 54-days-old (1, 3, 6, 15, 21 and 54 days). Despite all the researches on fish brain, there are no study on nerves evolution on H. huso during their larvae life. For this research 40 samples of larvae H. huso were obtained (from each age, about six samples were selected). The specimens were maintained in fiberglass t...

متن کامل

Cryopreservation of Beluga (Huso huso) Sperm: Effect of Different C

Semen obtained from four Beluga males (Huso huso) was cryopreserved using extender; Tris-sucroseKCl (30mM Tris, 23.4mM sucrose, 0.25mM KCl, PH 8.0) supplemented with MeOH at concentration of 5%, 10% and 20%. Semen was diluted, respectively, with ratios of 1:0.5, 1:1 and 1:2 with extender and frozen in liquid nitrogen vapor.Frozen sperms after 30 and 60 days were excluded from freezing. Experime...

متن کامل

Sublethal toxicity of organophosphate, diazinon on some organs of great sturgeon (Huso huso)

  Sublethal toxicity effects of diazinon on the structure of some organs of great sturgeon ( Huso huso ) were investigated. Diazinon was applied at a sublethal concentration of 1.5 mg L -1 at 22±1 ºC for nine weeks as a constant bath. Tissues of liver, kidney and gills were sampled and examined histopathologically   after 1, 14, 28, 42 and 63 days post exposure to the...

متن کامل

Lactococcus lactis subsp. lactis infection in Bester sturgeon, a cultured hybrid of Huso huso × Acipenser ruthenus, in Taiwan.

Approximately 5300 hybrid sturgeons with an average body weight of 600-800 g were farmed in 3 round tankers measuring 3m in diameter each containing 28,000 L of aerated groundwater. According to the owner's description, the diseased fish had anorexia, pale body color, and reddish spots on the abdomen. The morbidity and lethality rates in this outbreak were about 70% (3706/5300) and 100% (3706/3...

متن کامل

Mineral Status of Juvenile Beluga (Huso huso) Fed Citric Acid Supplemented Diets

A study was conducted to investigate the effects of dietary citric acid (CA) (0, 1, 2 and 3%) on the Calcium(Ca) and Phosphorus(P) content of muscle, scute and serum of Beluga (Huso huso) juveniles (25.1±1.9 g). After 8 weeks feeding on the experimental diets, Calcium(Ca) and Phosphorus(P) content of muscle, scute and serum were measured. The results revealed that addition with 2% and 3% CA sig...

متن کامل

Effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (Huso huso) spermatozoa.

Broodstock selection programs are currently underway for sturgeon species. To complement and further these selection programs we need to develop sperm cryopreservation procedures. In the present study, we describe the effects of freezing rate (-10°C, -15°C, -20°C, -30°C and -40°C/min) on gamete quality characteristics (i.e., duration of motility (s), motility percentage (%), ATP content (nmol/1...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
بهره برداری و پرورش آبزیان

جلد ۳، شماره ۱، صفحات ۱۷-۳۴

کلمات کلیدی
[ ' i n p r e s e n t s t u d y ' , ' i t w a s d e t e r m i n a t e d l c 5 0 9 6 ' , ' h a n d t o x i c i t y d e g r e e o f d i a z i n o n i n h u s o h u s o ' , ' t h e n w e r e e v a l u a t e d e f f e c t s o f s u b ' , ' l e t h a l c o n c e n t r a t i o n s o n t o w g e n e r a l e n z y m a t i c i n d i c e s i n c l u d i n g a l a n i n e a n d a s p a r t a t e a m i n o t r a n s f e r a s e o f p l a s m a a n d o n e s p e c i f i c e n z y m a t i c i n d e x i n c l u d i n g t i s s u e a c h e e n z y m e . t h e m e d i a n l e t h a l c o n c e n t r a t i o n ' , ' l o w e s t o b s e r v e d e f f e c t c o n c e n t r a t i o n a n d m a x i m u m a l l o w a b l e t o x i c a n t c o n c e n t r a t i o n w a s m e a s u r e d 5 . 4 7 ' , ' 4 . 2 8 a n d 0 . 5 4 m g . l ' , 1 , ' r e s p e c t i v e l y . a l s o ' , ' d i a z i n o n a c c o r d i n g b y a m o u n t o f l c 5 0 w a s c l a s s i f i e d i n “ m o d e r a t e t o x i c i t y d e g r e e ” f o r t h i s s t u r g e o n . t h e t e s t r e s u l t s s h o w e d t h a t w i t h i n c r e a s i n g s u b ' , ' l e t h a l c o n c e n t r a t i o n s o f d i a z i n o n i n w a t e r ' , ' a s t a n d a l t e n z y m e a c t i v i t y i n e x p e r i m e n t a l f i s h w e r e i n c r e a s e d ' , ' t h u s ' , ' a d i r e c t r e l a t i o n s h i p s e e n b e t w e e n t h e c o n c e n t r a t i o n o f d i a z i n o n w i t h t h e p u b l i c i n d i c e s . h o w e v e r ' , ' i n c r e a s e d c o n c e n t r a t i o n s o f d i a z i n o n ' , ' c a u s i n g a d e c r e a s e i n c h o l i n e s t e r a s e a c t i v i t y i n b o t h b r a i n a n d m u s c l e t i s s u e s . i n o t h e r w o r d s ' , ' i n h i b i t i o n o f a c h e i s d i r e c t l y r e l a t e d t o t h e c o n c e n t r a t i o n o f p e s t i c i d e . t h e d i a z i n o n p e s t i c i d e c o n c e n t r a t i o n s f o r n o n ' , ' t a r g e t s p e c i e s e s w h i c h w e r e i n e f f e c t i v e a n d a l l o w a b l e a c c o r d i n g t o t h e l e t h a l t e s t c a n l e a d t o t h e i r e n z y m a t i c i n d i c i e s o f s e r u m a n d a c h e r e s p o n s e s . ' ]

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023